Schedule 8

EIGHTH SCHEDULERegulation 28(2)Part 1Permitted general purpose food additivesAcetic acid;Acetone;Adipic acid;Ammonium bicarbonate;Ammonium carbonate;Ammonium hydroxide;Ammonium phosphate (mono- and di-basic);Ammonium sulphate;Aspartame;Beeswax;Calcium carbonate;Calcium chloride;Calcium citrate;Calcium gluconate;Calcium hydroxide;Calcium lactate;Calcium phosphate (mono-, di-and tri-basic);Calcium sulphate;Candelilla wax;Carbon dioxide;Carnauba wax;Citric acid;Cyclotetraglucose;Cyclotetraglucose syrup;Erythritol;Fumaric acid;Glucono delta-lactone;Glycerin or Glycerol;Glycerol esters of citric, lactic and tartaric acids;Helium;High fructose syrup;Hydrochloric acid;Hydrogen peroxide;Hydrogenated starch hydrolysate (polyglycitol syrup);Isomalt;Lactic acid;Lactitol;Magnesium carbonate;Magnesium chloride;Magnesium oxide;Magnesium hydroxide;Magnesium hydroxide carbonate;Magnesium lactate;Magnesium stearate;Magnesium sulphate;Malic acid;Maltitol;Maltitol syrup (hydrogenated glucose syrup);Mannitol;Nitrogen;Nitrous oxide;Oligofructose (from chicory root);Peracetic acid;Phosphoric acid;Polydextrose;Polyethylene glycol;Polyvinylpyrrolidone, insoluble (polyvinylpolypyrrolidone);Potassium acid tartrate;Potassium bicarbonate;Potassium carbonate;Potassium chloride;Potassium citrate;Potassium hydroxide;Potassium sulphate;Propylene glycol;Pullulan;Shellac;Silica aerogel;Sodium acetate;Sodium acid sulphate (sodium hydrogen sulphate);Sodium chloride;Sodium aluminium phosphate;Sodium bicarbonate;Sodium carbonate;Sodium citrate;Sodium fumarate;Sodium hydroxide;Sodium phosphate (mono-, di-and tri-basic);Sodium potassium tartrate;Sodium pyrophosphate;Sodium sesquicarbonate;Sodium sulphate;Sodium tartrate;Sorbitol;Sorbitol syrup;Succinic acid;Sucrose esters of fatty acids;Sucrose oligoesters, type I and type II;Sulphuric acid;Sulphurous acid;Tartaric acid;Thaumatin;Trehalose;Triammonium citrate;Xylitol.[S 59/2019 wef 01/02/2019]Part 2PERMITTED ENZYMES(A)Enzymes derived from animal sourcesEnzymeEC NumberSourceCatalase1.11.1.6Bovine liverLactoperoxidase1.11.1.7Bovine milkLipase, triacylglycerol3.1.1.3Bovine stomach; salivary glands or forestomach of calf, kid or lamb; porcine or bovine pancreasLysozyme3.2.1.17Egg whitesPancreatin (or pancreatic elastase)3.4.21.36Pancreas of the hog or ox Pepsin3.4.23.1Bovine or porcine stomachPhospholipase A23.1.1.4Porcine pancreasRennet3.4.23.4Aqueous extracts from the fourth stomach of calves, kids, lambs, and adult bovine animals, sheep and goatsThrombin3.4.21.5Bovine or porcine bloodTrypsin3.4.21.4Porcine or bovine pancreas(B)Enzymes derived from plant sourcesEnzymeEC NumberSourceAlpha-amylase3.2.1.1Malted cerealsActinidin3.4.22.14Kiwifruit (Actinidia deliciosa)Beta-amylase3.2.1.2Malted cereals Sweet potato (Ipomoea batatas)Bromelain3.4.22.4Pineapple fruit/stem (Ananas comosus and Ananas bracteatus (L))Ficin3.4.22.3Ficus spp.Lipoxidase1.13.11.12Soyabean whey or mealPapain3.4.22.2Carica papaya (L) (Fam. Caricaceae)(C)Enzymes derived from microbial sourcesEnzymeEC NumberProduction organismDonor organismDonor gene1,4-alpha-glucan branching enzyme2.4.1.18Bacillus subtilisRhodothermus obamensis1,4‑alpha‑glucan branching enzyme Geobacillus stearothermophilus2 Alpha-acetolactate decarboxylase4.1.1.5Bacillus amyloliquefaciens Bacillus licheniformisBacillus brevisAlpha-acetolactate decarboxylase Bacillus subtilis Bacillus subtilisBacillus brevisAlpha-acetolactate decarboxylaseAlpha, alpha‑trehalase3.2.1.28Trichoderma longibrachiatum3Trichoderma longibrachiatum3Alpha, alpha‑trehalaseAlpha-amylase3.2.1.1Aspergillus niger1 Aspergillus niger1Aspergillus niger1Alpha-amylase Aspergillus niger1Rhizomucor pusillusAlpha-amylase Aspergillus oryzae Bacillus amyloliquefaciens Bacillus amyloliquefaciensBacillus amyloliquefaciensAlpha-amylase Bacillus licheniformis Bacillus licheniformisBacillus amyloliquefaciensAlpha-amylase Bacillus licheniformisBacillus licheniformisAlpha-amylase Bacillus licheniformisBacillus licheniformis and Bacillus amyloliquefaciensAlpha-amylase Bacillus licheniformisGeobacillus stearothermophilus2Alpha-amylase Bacillus licheniformisPseudomonas stutzeriAlpha-amylase Bacillus subtilis Bacillus subtilisBacillus megateriumAlpha-amylase Bacillus subtilis Alicyclobacillus pohliaeAlpha-amylase Bacillus subtilisGeobacillus stearothermophilus2Alpha-amylase Geobacillus stearothermophilus2 Geobacillus stearothermophilus2Geobacillus stearothermophilus2Alpha-amylase Microbacterium imperiale Pseudomonas fluorescensThermococcalesAlpha-amylase Rhizopus oryzae Trichoderma longibrachiatum3Aspergillus kawachiiAlpha-amylaseAlpha-arabinofuranosidase3.2.1.55Aspergillus niger1 Alpha-galactosidase3.2.1.22Aspergillus niger1 Alpha-glucosidase (or maltase)3.2.1.20Aspergillus niger1 Aspergillus oryzae Trichoderma longibrachiatum3Aspergillus niger1Alpha-glucosidase (or maltase)Aminopeptidase3.4.11.1Aspergillus oryzae Lactocococcus lactis Amylomaltase2.4.1.25Bacillus amyloliquefaciensThermus thermophilusAmylomaltaseAqualysin 13.4.21.111Bacillus subtilisThermus aquaticusAqualysin 1Asparaginase3.5.1.1Aspergillus niger1 Aspergillus niger1Aspergillus niger1Asparaginase Aspergillus oryzae Aspergillus oryzaeAspergillus oryzaeAsparaginase Bacillus subtilisPyrococcus furiosusAsparaginaseAspergillopepsin I3.4.23.18Aspergillus niger1 Aspergillus oryzae Trichoderma longibrachiatum3Trichoderma longibrachiatum3Aspergillopepsin IAspergillopepsin II3.4.23.19Aspergillus niger1 Beta-amylase3.2.1.2Bacillus amyloliquefaciens Bacillus licheniformisBacillus flexusBeta-amylase Bacillus subtilis Beta-fructofuranosidase (invertase or saccharase)3.2.1.26Aspergillus japonicus Aspergillus niger1 Saccharomyces cerevisiae Beta-galactosidase (or lactase)3.2.1.23Aspergillus niger1 Aspergillus niger1Aspergillus oryzaeBeta-galactosidase (or lactase) Aspergillus oryzae Bacillus circulans ATCC 31382 Bacillus licheniformisBifidobacterium bifidumBeta-galactosidase (or lactase) Bacillus subtilisBifidobacterium bifidumBeta-galactosidase (or lactase) Kluyveromyces lactis4 Kluyveromyces marxianus5 Saccharomyces sp. Beta-glucanase (endo-beta glucanase or endo-1,3-beta-glucanase)3.2.1.6Aspergillus niger1 Aspergillus oryzae Bacillus amyloliquefaciens Bacillus amyloliquefaciensBacillus amyloliquefaciensBeta-glucanase (endo-beta glucanase or endo-1,3-beta-glucanase) Bacillus subtilis Bacillus subtilisBacillus subtilisBeta-glucanase (endo-beta glucanase or endo-1,3-beta-glucanase) Disporotrichum dimorphosphorum Humicola insolens Rasamsonia emersonii6 Trichoderma longibrachiatum3 Beta-glucosidase3.2.1.21Aspergillus niger1 Carboxylesterase3.1.1.1Rhizomucor miehei7 Carboxypeptidase C3.4.16.5Aspergillus niger1Aspergillus niger1Carboxypeptidase CCatalase1.11.1.6Aspergillus niger1 Aspergillus niger1Aspergillus niger1Catalase Micrococcus luteus8 Cellulase3.2.1.4Aspergillus niger1 Penicillium funiculosum Rasamsonia emersonii6 Trichoderma longibrachiatum3 Trichoderma longibrachiatum3Trichoderma longibrachiatum3Cellulase Trichoderma viride Chymosin3.4.23.4Aspergillus niger1 Aspergillus niger var. awamori Camelus dromedariusChymosin Escherichia coli K-12 strain GE81 Kluyveromyces lactis4 Kluyveromyces lactis4Calf prochymosin BChymosin Cyclodextrin glucanotransferase2.4.1.19Bacillus licheniformisThermoanaerobacter sp.Cyclodextrin glucanotransferase Paenibacillus macerans9 Deaminase3.5.4.6Aspergillus melleus Dextranase3.2.1.11Chaetomium erraticum Chaetomium gracile Penicillium lilacinum Endo-arabinase3.2.1.99Aspergillus niger1 Endo-protease 3.4.21.26Aspergillus niger1 Aspergillus niger1Aspergillus niger1Endo-proteaseGlucan 1,3-beta-glucosidase3.2.1.58Trichoderma harzianum Endo-1,3-beta-xylanase3.2.1.32Humicola insolens Endo-1,4-beta-xylanase3.2.1.8Aspergillus niger1 Aspergillus niger1Aspergillus niger1Endo-1,4-beta-xylanase Aspergillus niger1Rasamsonia emersonii6Endo-1,4-beta-xylanase Aspergillus oryzae Aspergillus oryzaeAspergillus aculeatusEndo-1,4-beta-xylanase Aspergillus oryzaeHumicola lanuginosa10Endo-1,4-beta-xylanase Bacillus amyloliquefaciens Bacillus licheniformisBacillus licheniformisEndo-1,4-beta-xylanase Bacillus subtilis Bacillus subtilisBacillus subtilisEndo-1,4-beta-xylanase Bacillus subtilisPseudoalteromonas haloplanktisEndo-1,4-beta-xylanase Disporotrichum dimorphosphorum Humicola insolens Rasamsonia emersonii6 Trichoderma longibrachiatum3 Trichoderma longibrachiatum3Aspergillus niger1Endo-1,4-beta-xylanase Trichoderma longibrachiatum3Aspergillus tubingensisEndo-1,4-beta-xylanase Trichoderma longibrachiatum3Thermopolyspora flexuosa11Endo-1,4-beta-xylanaseGlucoamylase (or amyloglucosidase)3.2.1.3Aspergillus niger1 Aspergillus niger1Aspergillus niger1Glucoamylase (or amyloglucosidase) Aspergillus niger1Gloeephyllum trabeumGlucoamylase (or amyloglucosidase) Aspergillus niger1Penicillum oxalicumGlucoamylase (or amyloglucosidase) Aspergillus niger1Talaromyces emersoniiGlucoamylase (or amyloglucosidase) Aspergillus niger1Trametes cingulataGlucoamylase (or amyloglucosidase) Aspergillus oryzae Rhizopus delemar Rhizopus niveus Rhizopus oryzae Trichoderma longibrachiatum3Trichoderma longibrachiatum3Glucoamylase (or amyloglucosidase)Glucose isomerase (or xylose isomerase)5.3.1.5Actinoplanes missouriensis Bacillus coagulans Microbacterium arborescens Streptomyces olivaceus Streptomyces olivochromogenes Streptomyces murinus Streptomyces rubiginosus Streptomyces rubiginosusStreptomyces rubiginosusGlucose isomerase (or xylose isomerase)Glucose oxidase1.1.3.4Aspergillus niger1 Aspergillus niger1Aspergillus niger1Glucose oxidase Aspergillus niger1Penicillium chrysogenumGlucose oxidase Aspergillus oryzaeAspergillus niger1Glucose oxidase Penicillium chrysogenum Glutaminase3.5.1.2Bacillus amyloliquefaciens Glycerophospholipid cholesterol acyltransferase2.3.1.43Bacillus licheniformisAeromonas salmonicida subsp. salmonicidaGlycerophospholipid cholesterol acyltransferaseHexose oxidase1.1.3.5Hansenula polymorpha12Chondrus crispusHexose oxidaseInulinase3.2.1.7Aspergillus niger1 Lipase, monoacylglycerol3.1.1.23Penicillium camembertii Lipase, triacylglycerol3.1.1.3Aspergillus niger1 Aspergillus niger1Candida antarcticaLipase, triacylglycerol Aspergillus niger1Fusarium culmorumLipase, triacylglycerol Aspergillus oryzae Aspergillus oryzaeFusarium oxysporumLipase, triacylglycerol Aspergillus oryzaeHumicola lanuginosa10Lipase, triacylglycerol Aspergillus oryzaeRhizomucor miehei7Lipase, triacylglycerol Aspergillus oryzaeHumicola lanuginosa10 and Fusarium oxysporumLipase, triacylglycerol Candida rugosa Hansenula polymorpha12Fusarium heterosporumLipase, triacylglycerol Mucor javanicus13 Penicillium roquefortii14 Rhizomucor miehei7 Rhizopus arrhizus Rhizopus niveus Rhizopus oryzae Lysophospholipase3.1.1.5Aspergillus niger1 Aspergillus niger1Aspergillus niger1Lysophospholipase Trichoderma longibrachiatum3Aspergillus nishimuraeLysophospholipaseMaltogenic alpha-amylase 3.2.1.133Bacillus licheniformisGeobacillus stearothermophilus2Maltogenic alpha-amylase Bacillus subtilisGeobacillus stearothermophilus2Maltogenic alpha-amylaseMaltotetraohydrolase3.2.1.60Bacillus licheniformisPseudomonas stutzeriMaltotetraohydrolaseMannan endo-1,4-beta-mannosidase3.2.1.78Aspergillus niger1 Bacillus amyloliquefaciens Bacillus subtilis Trichoderma longibrachiatum3 Trichoderma longibrachiatum3Trichoderma longibrachiatum3Mannan endo-1,4-beta-mannosidaseMetalloproteinase153.4.24.4Aspergillus oryzae Bacillus amyloliquefaciens Bacillus amyloliquefaciensBacillus amyloliquefaciensMetalloproteinase Bacillus coagulans Bacillus subtilis Bacillus subtilisBacillus amyloliquefaciensMetalloproteinase Geobacillus caldoproteolyticus Geobacillus stearothermophilus2 Mucorpepsin (or aspartic proteinase)3.4.23.23Aspergillus oryzae Aspergillus oryzaeRhizomucor miehei7Aspartic proteinase Cryphonectria parasitica Rhizomucor miehei7 Pectin esterase3.1.1.11Aspergillus niger1 Aspergillus niger1Aspergillus niger1Pectin esterase Aspergillus oryzaeAspergillus aculeatusPectin esterasePectin lyase4.2.2.10Aspergillus niger1 Aspergillus niger1Aspergillus niger1Pectin lyase Trichoderma longibrachiatum3Aspergillus niger1Pectin lyasePeroxidase1.11.1.7Aspergillus niger1Marasmius scorodoniusPeroxidasePhosphatidylinositol phospholipase C3.1.4.11Pseudomonas fluorescensIsolated from soilPhosphatidylinositol phospholipase CPhosphodiesterase I3.1.4.1Leptographium procerum Phospholipase A13.1.1.32Aspergillus oryzaeFusarium venenatumPhospholipase A1Phospholipase A23.1.1.4Aspergillus niger1Porcine pancreasPhospholipase A2 Streptomyces violaceoruber Trichoderma longibrachiatum3Aspergillus nishimuraePhospholipase A2Phospholipase C3.1.4.3Pichia pastorisIsolated from soilPhospholipase C3-Phytase3.1.3.8Aspergillus niger1 Aspergillus niger1Aspergillus niger13-Phytase4-Phytase3.1.3.26Aspergillus oryzaePeniophora lycii4-PhytasePolygalacturonase (pectinase) 3.2.1.15Aspergillus niger1 Aspergillus niger1Aspergillus niger1Polygalacturonase (pectinase) Aspergillus oryzae Aspergillus oryzaeAspergillus aculeatusPolygalacturonase (pectinase) Rhizopus oryzae Trichoderma longibrachiatum3 Protein-glutaminase3.5.1.44Chryseobacterium proteolyticum Pullulanase3.2.1.41Bacillus acidopullulyticus Bacillus amyloliquefaciens Bacillus licheniformis Bacillus licheniformis Bacillus deramificansPullulanase Bacillus subtilis Bacillus subtilisBacillus acidopullulyticusPullulanase Bacillus subtilisBacillus deramificansPullulanase Bacillus subtilisBacillus naganoensisPullulanase Klebsiella pneumoniae16 Pullulanibacillus sp. Ribonuclease3.1.26.5Penicillium citrinum Serine proteinase173.4.21.14Aspergillus melleus Aspergillus oryzae Bacillus amyloliquefaciens Bacillus halodurans Bacillus licheniformis Bacillus subtilis Serine protease (Chymotrypsin)3.4.21.1Bacillus licheniformisNocardiopsis prasinaSerine protease (Chymotrypsin)Serine protease with trypsin specificity3.4.21.4Fusarium venenatumFusarium oxysporumSerine protease with trypsin specificityTannase3.1.1.20Aspergillus oryzae Transglucosidase2.4.1.24Aspergillus niger1 Trichoderma longibrachiatum3Aspergillus niger1TransglucosidaseTransglutaminase2.3.2.13Streptomyces mobaraensis18 Urease3.5.1.5Lactobacillus fermentum [S 59/2019 wef 01/02/2019]1 Aspergillus niger group includes A. aculeatus, A. awamori, A. ficuum, A. foetidus, A. japonicus, A. phoenicis, A. saitor and A. usamii.2 Geobacillus stearothermophilus – former name Bacillus stearothermophilus.3 Trichoderma longibrachiatum also known as Trichoderma reesei.4 Kluyveromyces lactis – former name Saccharomyces lactis.5 Kluyveromyces marxianus – former names Saccharomyces fragilis and Kluyveromyces fragilis.6 Rasamsonia emersonii – former name Talaromyces emersonii.7 Rhizomucor miehei – former name Mucor miehei.8 Micrococcus luteus – former name Micrococcus lysodeikticus.9 Paenibacillus macerans – former name Bacillus macerans.10 Humicola lanuginosa also known as Thermomyces lanuginosus.11 Thermopolyspora flexuosa – former name Nonomuraea flexuosa.12 Hansenula polymorpha also known as Pichia angusta.13 Mucor javanicus also known as Mucor circinelloides f. circinelloides.14 Penicillium roquefortii also known as Penicillium roqueforti.15 Metalloproteinase (EC 3.4.24.4) includes vibriolysin (EC 3.4.24.25), pseudolysin (EC 3.4.24.26), thermolysin (3.4.24.27), bacillolysin (EC 3.4.24.28), aureolysin (EC 3.4.24.29), coccolysin (EC 3.4.24.30), mycolysin (EC 3.4.24.31), beta-lytic metalloendopeptidase (EC 3.4.24.32), deuterolysin (EC.3.4.24.39), serralysin (EC 3.4.24.40).16 Klebsiella pneumoniae – former name Klebsiella aerogenes.17 Serine proteinase (EC 3.4.21.14) includes oryzin (EC 3.4.21.63) and subtilisin (EC 3.4.21.62).18 Streptomyces mobaraensis – former name Streptoverticillium mobaraense.[S 59/2019 wef 01/02/2019][S 152/2017 wef 01/04/2017][S 816/2014 wef 15/12/2014][S 493/2013 wef 01/08/2013][S 444/2012 wef 03/09/2012][S 195/2011 wef 15/04/2011][S 704/2020 wef 31/08/2020]